Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

Genetic imprinting ensures monoallelic gene expression critical for normal embryonic development. Here the authors take advantage of human haploid parthenogenic embryonic stem cells lacking paternal alleles to identify, by genome-wide screening, factors involved in the regulation of imprinted genes.

Bibliographic Details
Main Authors: Shiran Bar, Dan Vershkov, Gal Keshet, Elyad Lezmi, Naama Meller, Atilgan Yilmaz, Ofra Yanuka, Malka Nissim-Rafinia, Eran Meshorer, Talia Eldar-Geva, Nissim Benvenisty
Format: Article
Language:English
Published: Nature Portfolio 2021-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-26949-7

Internet

https://doi.org/10.1038/s41467-021-26949-7

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