An improved RT-qPCR method for direct quantification of enveloped RNA viruses

Reverse transcription quantitative PCR (RT-qPCR) has emerged as the gold standard for virus detection and quantification, being utilized in numerous diagnostic and research applications. However, the direct detection of viruses has so far posed a challenge as the viral genome is often encapsidated b...

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Bibliographic Details
Main Authors: Pavlina Gregorova, Minna-Maria K. Heinonen, L. Peter Sarin
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016122001182