Protein Engineering of <i>Pasteurella multocida</i> α2,3-Sialyltransferase with Reduced α2,3-Sialidase Activity and Application in Synthesis of 3′-Sialyllactose

Protein Engineering of <i>Pasteurella multocida</i> α2,3-Sialyltransferase with Reduced α2,3-Sialidase Activity and Application in Synthesis of 3′-Sialyllactose

Sialyltransferases are key enzymes for the production of sialosides. The versatility of <i>Pasteurella multocida</i> α2,3-sialyltransferase 1 (PmST1) causes difficulties in the efficient synthesis of α2,3-linked sialylatetd compounds, especial its α2,3-sialidase activity. In the current...

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Bibliographic Details
Main Authors: Rui Yang, Mengge Gong, Siming Jiao, Juntian Han, Cui Feng, Meishan Pei, Zhongkai Zhou, Yuguang Du, Jianjun Li
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Catalysts
Subjects:
sialyltransferases
<i>Pasteurella multocida</i> α2,3-sialyltransferase
α2,3-sialidase
kinetic characterization
3′-sialyllactose
Online Access:https://www.mdpi.com/2073-4344/12/6/579

Internet

https://www.mdpi.com/2073-4344/12/6/579

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