N-hydroxysuccinimidyl derivatization reagents, friends or foes? From synthesis to derivatization of amino compounds and their analysis using liquid chromatography tandem mass spectrometry
Abstract N-Hydroxysuccinimidyl (NHS) esters are widely employed in peptide coupling; however, their behavior in derivatization for high-performance liquid chromatography (HPLC) analysis differs from the synthesis point of view. This work evaluated the performance of NHS derivatization reagents for a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-02-01
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| Series: | Discover Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s42452-025-06554-9 |