Establishment of an Efficient Immortalization Strategy Using HMEJ-Based b<i>TERT</i> Insertion for Bovine Cells

Establishment of an Efficient Immortalization Strategy Using HMEJ-Based b<i>TERT</i> Insertion for Bovine Cells

Immortalized cell lines have been used in a wide range of applications in research on immune disorders and cellular metabolic regulation due to the stability and uniformity of their cellular characteristics. At present, the investigation into molecular functions and signaling pathways within bovine...

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Bibliographic Details
Main Authors: Zihan Zhang, Zhuo Han, Ying Guo, Xin Liu, Yuanpeng Gao, Yong Zhang
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:International Journal of Molecular Sciences
Subjects:
immortalization
bovine
telomerase reverse transcriptase (<i>TERT</i>)
homology-mediated end joining (HMEJ)
CRISPR/Cas9
macrophage
Online Access:https://www.mdpi.com/1422-0067/22/22/12540

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https://www.mdpi.com/1422-0067/22/22/12540

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