Protocol for CRISPR-based endogenous protein tagging in mammalian cells

Protocol for CRISPR-based endogenous protein tagging in mammalian cells

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Summary: Tracking the localization and proximal interaction partners of endogenous proteins provides valuable functional insight. Here, we present a protocol for CRISPR-based endogenous protein tagging in mammalian cells. We describe steps for endogenously tagging human TSC22D2 and MAP4, including d...

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Bibliographic Details
Main Authors: Yu-Xi Xiao, Jiarun Wei, Jason Moffat
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Genetics
High-Throughput Screening
Microscopy
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005690

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http://www.sciencedirect.com/science/article/pii/S2666166724005690

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