AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities.

High throughput sequencing (HTSeq) of small ribosomal subunit amplicons has the potential for a comprehensive characterization of microbial community compositions, down to rare species. However, the error-prone nature of the multi-step experimental process requires that the resulting raw sequences a...

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Bibliographic Details
Main Authors: Anja Lange, Steffen Jost, Dominik Heider, Christina Bock, Bettina Budeus, Elmar Schilling, Axel Strittmatter, Jens Boenigk, Daniel Hoffmann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4629888?pdf=render