AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities.
High throughput sequencing (HTSeq) of small ribosomal subunit amplicons has the potential for a comprehensive characterization of microbial community compositions, down to rare species. However, the error-prone nature of the multi-step experimental process requires that the resulting raw sequences a...
Main Authors: | Anja Lange, Steffen Jost, Dominik Heider, Christina Bock, Bettina Budeus, Elmar Schilling, Axel Strittmatter, Jens Boenigk, Daniel Hoffmann |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4629888?pdf=render |
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