An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination

An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination

Previously, we described a large collection of Drosophila strains that each carry an artificial exon containing a T2AGAL4 cassette inserted in an intron of a target gene based on CRISPR-mediated homologous recombination. These alleles permit numerous applications and have proven to be very useful. I...

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Bibliographic Details
Main Authors: Oguz Kanca, Jonathan Zirin, Yanhui Hu, Burak Tepe, Debdeep Dutta, Wen-Wen Lin, Liwen Ma, Ming Ge, Zhongyuan Zuo, Lu-Ping Liu, Robert W Levis, Norbert Perrimon, Hugo J Bellen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2022-06-01
Series:eLife
Subjects:
CRISPR
homologous recombination
knock-in
knock-out
gene trap
protein trap
Online Access:https://elifesciences.org/articles/76077

Internet

https://elifesciences.org/articles/76077

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