Challenges of Comparing Marine Microbiome Community Composition Data Provided by Different Commercial Laboratories and Classification Databases

Challenges of Comparing Marine Microbiome Community Composition Data Provided by Different Commercial Laboratories and Classification Databases

In the high-throughput sequencing (HTS) era, a metabarcoding technique based on the bacterial V3–V4 hypervariable region of 16S rRNA analysis requires sophisticated bioinformatics pipelines and validated methods that allow researchers to compare their data with confidence. Many commercial laboratori...

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Bibliographic Details
Main Authors: Monika Mioduchowska, Anna Iglikowska, Jan P. Jastrzębski, Anna-Karina Kaczorowska, Ewa Kotlarska, Artur Trzebny, Agata Weydmann-Zwolicka
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Water
Subjects:
amplicon sequencing
ASV assignment
HTS
marine microorganisms
metagenetics
“mock community”
Online Access:https://www.mdpi.com/2073-4441/14/23/3855

Internet

https://www.mdpi.com/2073-4441/14/23/3855

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