A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs
Neonatal alloimmune thrombocytopenia results from the maternal immune response against fetal-specific antigens inherited from the father. The diagnosis is ascertained only when the maternal alloantibody and the offending antigen present in the newborn are identified. Up until now most laboratories p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | International Journal of Neonatal Screening |
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| Online Access: | http://www.mdpi.com/2409-515X/2/3/3 |
| _version_ | 1811326373303681024 |
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| author | Gérald Bertrand Cécile Kaplan |
| author_facet | Gérald Bertrand Cécile Kaplan |
| author_sort | Gérald Bertrand |
| collection | DOAJ |
| description | Neonatal alloimmune thrombocytopenia results from the maternal immune response against fetal-specific antigens inherited from the father. The diagnosis is ascertained only when the maternal alloantibody and the offending antigen present in the newborn are identified. Up until now most laboratories perform DNA extraction for neonatal genotyping from newborn blood samplings. In order to avoid such an invasive procedure, two protocols of DNA extraction from buccal swabs were developed: a manual protocol using the QIAamp mini blood kit (Qiagen), and an automated procedure with the MagNA Pure Compact instrument (Roche). Both EDTA-blood and buccal swabs from thrombocytopenic newborns were genotyped manually (14 samples), automatically (15 samples) or both manually and automatically (two samples). Human Platelet Antigen (HPA) genotyping was performed using the BeadChip assay (BioArray, Immucor). Concordant genotypings were obtained for all samples except for one swab with the manual method. The automated DNA extraction from newborn buccal swabs with the MagNA Pure Compact instrument was chosen as the first-line strategy, with a significant gain of time in processing buccal swabs. |
| first_indexed | 2024-04-13T14:48:32Z |
| format | Article |
| id | doaj.art-21499ce36e114cf88e68e892488060de |
| institution | Directory Open Access Journal |
| issn | 2409-515X |
| language | English |
| last_indexed | 2024-04-13T14:48:32Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Neonatal Screening |
| spelling | doaj.art-21499ce36e114cf88e68e892488060de2022-12-22T02:42:40ZengMDPI AGInternational Journal of Neonatal Screening2409-515X2016-07-0123310.3390/ijns2030003ijns2030003A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal SwabsGérald Bertrand0Cécile Kaplan1EFS Bretagne, HLA-HPA Laboratory, Rue Pierre-Jean Gineste, BP91614, 35016 Rennes Cedex, FrancePlatelet Immunology Laboratory, Institut National de la Transfusion Sanguine (INTS), 75739 Paris, FranceNeonatal alloimmune thrombocytopenia results from the maternal immune response against fetal-specific antigens inherited from the father. The diagnosis is ascertained only when the maternal alloantibody and the offending antigen present in the newborn are identified. Up until now most laboratories perform DNA extraction for neonatal genotyping from newborn blood samplings. In order to avoid such an invasive procedure, two protocols of DNA extraction from buccal swabs were developed: a manual protocol using the QIAamp mini blood kit (Qiagen), and an automated procedure with the MagNA Pure Compact instrument (Roche). Both EDTA-blood and buccal swabs from thrombocytopenic newborns were genotyped manually (14 samples), automatically (15 samples) or both manually and automatically (two samples). Human Platelet Antigen (HPA) genotyping was performed using the BeadChip assay (BioArray, Immucor). Concordant genotypings were obtained for all samples except for one swab with the manual method. The automated DNA extraction from newborn buccal swabs with the MagNA Pure Compact instrument was chosen as the first-line strategy, with a significant gain of time in processing buccal swabs.http://www.mdpi.com/2409-515X/2/3/3neonatal alloimmune thrombocytopenia (NAIT)Human Platelet Antigen (HPA)genotypingbuccal swabDNA extraction |
| spellingShingle | Gérald Bertrand Cécile Kaplan A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs International Journal of Neonatal Screening neonatal alloimmune thrombocytopenia (NAIT) Human Platelet Antigen (HPA) genotyping buccal swab DNA extraction |
| title | A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs |
| title_full | A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs |
| title_fullStr | A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs |
| title_full_unstemmed | A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs |
| title_short | A Non-Invasive Strategy for Neonatal Alloimmune Thrombocytopenia Diagnosis: Newborn Platelet Genotyping with Buccal Swabs |
| title_sort | non invasive strategy for neonatal alloimmune thrombocytopenia diagnosis newborn platelet genotyping with buccal swabs |
| topic | neonatal alloimmune thrombocytopenia (NAIT) Human Platelet Antigen (HPA) genotyping buccal swab DNA extraction |
| url | http://www.mdpi.com/2409-515X/2/3/3 |
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