Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR

Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR

Summary: Gene-of-interest (GOI) knockout is an important technique to study the genetic mechanisms of T cells. Here, we present a protocol to generate GOI double allele gene knockouts in primary human T cells by CRISPR, thus depleting proteins of interest expressed intracellularly or extracellularly...

Full description

Bibliographic Details
Main Authors: Ling Wu, Jia Chi Tan, Nicholas R.J. Gascoigne
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Immunology
Molecular Biology
Gene Expression
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004124

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723004124

Similar Items