Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry

Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry

Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copies using Light Cycler® detection system and inte...

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Bibliographic Details
Main Authors: E. Kosinova, I. Psikal, B. Robesova, K. Kovarcik
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2007-06-01
Series:Veterinární Medicína
Subjects:
bovine viral diarrhea virus
rna
real-time rt-pcr
sybr green i
Online Access:https://vetmed.agriculturejournals.cz/artkey/vet-200706-0004_real-time-pcr-for-quantitation-of-bovine-viral-diarrhea-virus-rna-using-sybr-green-i-fluorimetry.php

Internet

https://vetmed.agriculturejournals.cz/artkey/vet-200706-0004_real-time-pcr-for-quantitation-of-bovine-viral-diarrhea-virus-rna-using-sybr-green-i-fluorimetry.php

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