Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copies using Light Cycler® detection system and inte...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2007-06-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200706-0004_real-time-pcr-for-quantitation-of-bovine-viral-diarrhea-virus-rna-using-sybr-green-i-fluorimetry.php |