Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation
The valorization of byproducts from the sugarcane industry represents a potential alternative method with a low energy cost for the production of metabolites that are of commercial and industrial interest. The production of exopolysaccharides (EPSs) was carried out using the yeast <i>Suhomyces...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/5/1105 |
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| author | Mariana González-Torres Francisco Hernández-Rosas Neith Pacheco Josafhat Salinas-Ruiz José A. Herrera-Corredor Ricardo Hernández-Martínez |
| author_facet | Mariana González-Torres Francisco Hernández-Rosas Neith Pacheco Josafhat Salinas-Ruiz José A. Herrera-Corredor Ricardo Hernández-Martínez |
| author_sort | Mariana González-Torres |
| collection | DOAJ |
| description | The valorization of byproducts from the sugarcane industry represents a potential alternative method with a low energy cost for the production of metabolites that are of commercial and industrial interest. The production of exopolysaccharides (EPSs) was carried out using the yeast <i>Suhomyces kilbournensis</i> isolated from agro-industrial sugarcane, and the products and byproducts of this agro-industrial sugarcane were used as carbon sources for their recovery. The effect of pH, temperature, and carbon and nitrogen sources and their concentration in EPS production by submerged fermentation (SmF) was studied in 170 mL glass containers of uniform geometry at 30 °C with an initial pH of 6.5. The resulting EPSs were characterized with Fourier-transform infrared spectroscopy (FT-IR). The results showed that the highest EPS production yields were 4.26 and 44.33 g/L after 6 h of fermentation using sucrose and molasses as carbon sources, respectively. Finally, an FT-IR analysis of the EPSs produced by <i>S. kilbournensis</i> corresponded to levan, corroborating its origin. It is important to mention that this is the first work that reports the production of levan using this yeast. This is relevant because, currently, most studies are focused on the use of recombinant and genetically modified microorganisms; in this scenario, <i>Suhomyces kilbournensis</i> is a native yeast isolated from the sugar production process, giving it a great advantage in the incorporation of carbon sources into their metabolic processes in order to produce levan sucrose, which uses fructose to polymerize levan. |
| first_indexed | 2024-04-25T00:23:01Z |
| format | Article |
| id | doaj.art-21921ec2736844b888700584cf38d142 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-25T00:23:01Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-21921ec2736844b888700584cf38d1422024-03-12T16:51:05ZengMDPI AGMolecules1420-30492024-02-01295110510.3390/molecules29051105Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged FermentationMariana González-Torres0Francisco Hernández-Rosas1Neith Pacheco2Josafhat Salinas-Ruiz3José A. Herrera-Corredor4Ricardo Hernández-Martínez5Colegio de Postgraduados, Campus Córdoba, Carretera Federal Córdoba-Veracruz Federal Km 348, Congregación Manuel León, Municipio Amatlán de los Reyes, Veracruz 94946, MexicoColegio de Postgraduados, Campus Córdoba, Carretera Federal Córdoba-Veracruz Federal Km 348, Congregación Manuel León, Municipio Amatlán de los Reyes, Veracruz 94946, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Subsede Sureste, Mérida 97302, MexicoColegio de Postgraduados, Campus Córdoba, Carretera Federal Córdoba-Veracruz Federal Km 348, Congregación Manuel León, Municipio Amatlán de los Reyes, Veracruz 94946, MexicoColegio de Postgraduados, Campus Córdoba, Carretera Federal Córdoba-Veracruz Federal Km 348, Congregación Manuel León, Municipio Amatlán de los Reyes, Veracruz 94946, MexicoCONAHCYT-Colegio de Postgraduados, Campus Córdoba, Carretera Federal Córdoba-Veracruz Federal Km 348, Congregación Manuel León, Municipio Amatlán de los Reyes, Veracruz 94946, MexicoThe valorization of byproducts from the sugarcane industry represents a potential alternative method with a low energy cost for the production of metabolites that are of commercial and industrial interest. The production of exopolysaccharides (EPSs) was carried out using the yeast <i>Suhomyces kilbournensis</i> isolated from agro-industrial sugarcane, and the products and byproducts of this agro-industrial sugarcane were used as carbon sources for their recovery. The effect of pH, temperature, and carbon and nitrogen sources and their concentration in EPS production by submerged fermentation (SmF) was studied in 170 mL glass containers of uniform geometry at 30 °C with an initial pH of 6.5. The resulting EPSs were characterized with Fourier-transform infrared spectroscopy (FT-IR). The results showed that the highest EPS production yields were 4.26 and 44.33 g/L after 6 h of fermentation using sucrose and molasses as carbon sources, respectively. Finally, an FT-IR analysis of the EPSs produced by <i>S. kilbournensis</i> corresponded to levan, corroborating its origin. It is important to mention that this is the first work that reports the production of levan using this yeast. This is relevant because, currently, most studies are focused on the use of recombinant and genetically modified microorganisms; in this scenario, <i>Suhomyces kilbournensis</i> is a native yeast isolated from the sugar production process, giving it a great advantage in the incorporation of carbon sources into their metabolic processes in order to produce levan sucrose, which uses fructose to polymerize levan.https://www.mdpi.com/1420-3049/29/5/1105levanexopolysaccharidescarbon sourcenitrogen source<i>Suhomyces kilbournensis</i> |
| spellingShingle | Mariana González-Torres Francisco Hernández-Rosas Neith Pacheco Josafhat Salinas-Ruiz José A. Herrera-Corredor Ricardo Hernández-Martínez Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation Molecules levan exopolysaccharides carbon source nitrogen source <i>Suhomyces kilbournensis</i> |
| title | Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation |
| title_full | Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation |
| title_fullStr | Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation |
| title_full_unstemmed | Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation |
| title_short | Levan Production by <i>Suhomyces kilbournensis</i> Using Sugarcane Molasses as a Carbon Source in Submerged Fermentation |
| title_sort | levan production by i suhomyces kilbournensis i using sugarcane molasses as a carbon source in submerged fermentation |
| topic | levan exopolysaccharides carbon source nitrogen source <i>Suhomyces kilbournensis</i> |
| url | https://www.mdpi.com/1420-3049/29/5/1105 |
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