Monolayer cell culture of freshly isolated adipocytes using extracellular basement membrane components.
Cell biological techniques requiring cells attached to surfaces, such as monolayer cell culture, microspectrofluorometry, and confocal microscopy, have not been readily available for use on adipocytes because they float and tend to lyse when attached to charged non-biological surfaces. A new method...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1995-04-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520400690 |