Electroporation of Mouse Follicles, Oocytes and Embryos without Manipulating Zona Pellucida
Electroporation is an effective technique of transfection, but its efficiency depends on the optimization of various parameters. In this study, a simplified and efficient method of gene manipulation was standardized through electroporation to introduce a recombinant green fluorescent protein (GFP) c...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Journal of Developmental Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2221-3759/9/2/13 |