A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly
Abstract Small extracellular–vesicule-associated microRNA (sEV-miRNA) is an important biomarker for cancer diagnosis. However, rapid and sensitive detection of low-abundance sEV-miRNA in clinical samples is challenging. Herein, a simple electrochemical biosensor that uses a DNA nanowire to localize...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02092-x |
| _version_ | 1797556700330328064 |
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| author | Wenbin Li Wen Wang Shihua Luo Siting Chen Tingting Ji Ningcen Li Weilun Pan Xiaohe Zhang Xiaojing Wang Ke Li Ye Zhang Xiaohui Yan |
| author_facet | Wenbin Li Wen Wang Shihua Luo Siting Chen Tingting Ji Ningcen Li Weilun Pan Xiaohe Zhang Xiaojing Wang Ke Li Ye Zhang Xiaohui Yan |
| author_sort | Wenbin Li |
| collection | DOAJ |
| description | Abstract Small extracellular–vesicule-associated microRNA (sEV-miRNA) is an important biomarker for cancer diagnosis. However, rapid and sensitive detection of low-abundance sEV-miRNA in clinical samples is challenging. Herein, a simple electrochemical biosensor that uses a DNA nanowire to localize catalytic hairpin assembly (CHA), also called domino-type localized catalytic hairpin assembly (DT-LCHA), has been proposed for sEV-miRNA1246 detection. The DT-LCHA offers triple amplification, (i). CHA system was localized in DNA nanowire, which shorten the distance between hairpin substrate, inducing the high collision efficiency of H1 and H2 and domino effect. Then, larger numbers of CHAs were triggered, capture probe bind DT-LCHA by exposed c sites. (ii) The DNA nanowire can load large number of electroactive substance RuHex as amplified electrochemical signal tags. (iii) multiple DT-LCHA was carried by the DNA nanowire, only one CHA was triggered, the DNA nanowire was trapped by the capture probe, which greatly improve the detection sensitivity, especially when the target concentration is extremely low. Owing to the triple signal amplification in this strategy, sEV-miRNA at a concentration of as low as 24.55 aM can be detected in 20 min with good specificity. The accuracy of the measurements was also confirmed using reverse transcription quantitative polymerase chain reaction. Furthermore, the platform showed good performance in discriminating healthy donors from patients with early gastric cancer (area under the curve [AUC]: 0.96) and was equally able to discriminate between benign gastric tumors and early cancers (AUC: 0.77). Thus, the platform has substantial potential in biosensing and clinical diagnosis. Graphical Abstract |
| first_indexed | 2024-03-10T17:05:38Z |
| format | Article |
| id | doaj.art-21eba5d4116c48f9add8cf674b758b92 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-10T17:05:38Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-21eba5d4116c48f9add8cf674b758b922023-11-20T10:48:10ZengBMCJournal of Nanobiotechnology1477-31552023-09-0121111210.1186/s12951-023-02092-xA sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assemblyWenbin Li0Wen Wang1Shihua Luo2Siting Chen3Tingting Ji4Ningcen Li5Weilun Pan6Xiaohe Zhang7Xiaojing Wang8Ke Li9Ye Zhang10Xiaohui Yan11Laboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityCenter for Clinical Laboratory Diagnosis and Research, The Affiliated Hospital of Youjiang Medical University for NationalitiesLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityLaboratory Medicine Center, Department of Laboratory Medicine, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical UniversityAbstract Small extracellular–vesicule-associated microRNA (sEV-miRNA) is an important biomarker for cancer diagnosis. However, rapid and sensitive detection of low-abundance sEV-miRNA in clinical samples is challenging. Herein, a simple electrochemical biosensor that uses a DNA nanowire to localize catalytic hairpin assembly (CHA), also called domino-type localized catalytic hairpin assembly (DT-LCHA), has been proposed for sEV-miRNA1246 detection. The DT-LCHA offers triple amplification, (i). CHA system was localized in DNA nanowire, which shorten the distance between hairpin substrate, inducing the high collision efficiency of H1 and H2 and domino effect. Then, larger numbers of CHAs were triggered, capture probe bind DT-LCHA by exposed c sites. (ii) The DNA nanowire can load large number of electroactive substance RuHex as amplified electrochemical signal tags. (iii) multiple DT-LCHA was carried by the DNA nanowire, only one CHA was triggered, the DNA nanowire was trapped by the capture probe, which greatly improve the detection sensitivity, especially when the target concentration is extremely low. Owing to the triple signal amplification in this strategy, sEV-miRNA at a concentration of as low as 24.55 aM can be detected in 20 min with good specificity. The accuracy of the measurements was also confirmed using reverse transcription quantitative polymerase chain reaction. Furthermore, the platform showed good performance in discriminating healthy donors from patients with early gastric cancer (area under the curve [AUC]: 0.96) and was equally able to discriminate between benign gastric tumors and early cancers (AUC: 0.77). Thus, the platform has substantial potential in biosensing and clinical diagnosis. Graphical Abstracthttps://doi.org/10.1186/s12951-023-02092-xDNA nanowireCatalyzed hairpin assemblysEV-miRNAsEarly cancer diagnostics |
| spellingShingle | Wenbin Li Wen Wang Shihua Luo Siting Chen Tingting Ji Ningcen Li Weilun Pan Xiaohe Zhang Xiaojing Wang Ke Li Ye Zhang Xiaohui Yan A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly Journal of Nanobiotechnology DNA nanowire Catalyzed hairpin assembly sEV-miRNAs Early cancer diagnostics |
| title | A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly |
| title_full | A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly |
| title_fullStr | A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly |
| title_full_unstemmed | A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly |
| title_short | A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly |
| title_sort | sensitive and rapid electrochemical biosensor for sev mirna detection based on domino type localized catalytic hairpin assembly |
| topic | DNA nanowire Catalyzed hairpin assembly sEV-miRNAs Early cancer diagnostics |
| url | https://doi.org/10.1186/s12951-023-02092-x |
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