Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains.

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid...

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Bibliographic Details
Main Authors: Linnéa Isaksson, Maxim Mayzel, Maria Saline, Anders Pedersen, Joakim Rosenlöw, Bernhard Brutscher, B Göran Karlsson, Vladislav Y Orekhov
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3647075?pdf=render