Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida
Hawthorn flavonoids were extracted by enzymolysis associated ultrasonic procedure. Thirteen flavonoids were identified by HPLC/ESI-QTOF/MS, and the major components were procyanidin C1, rutin-rhamnoside, vitexin-rhamnoside, and catechin. Hawthorn flavonoids exhibited strong free radical scavenging a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022023465 |
| _version_ | 1811192449626800128 |
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| author | Xin Huang Yuanyuan Bian Tianzhi Liu Zhuang Xu Ziqi Song Fei Wang Tuoping Li Suhong Li |
| author_facet | Xin Huang Yuanyuan Bian Tianzhi Liu Zhuang Xu Ziqi Song Fei Wang Tuoping Li Suhong Li |
| author_sort | Xin Huang |
| collection | DOAJ |
| description | Hawthorn flavonoids were extracted by enzymolysis associated ultrasonic procedure. Thirteen flavonoids were identified by HPLC/ESI-QTOF/MS, and the major components were procyanidin C1, rutin-rhamnoside, vitexin-rhamnoside, and catechin. Hawthorn flavonoids exhibited strong free radical scavenging activities against DPPH, ABTS, and hydroxyl radicals. Total and intercellular antioxidant experiments revealed that the free hydro-PSC value was 295.32 ± 12.20 μmol of VCE/g DW, and the free cellular antioxidant activity (CAA) values were 168.60 ± 4.87 μmol of QE/g DW in the no PBS wash protocol and 49.53 ± 1.75 μmol of QE/g DW in the PBS wash protocol. In addition, hawthorn flavonoids exhibited higher α-amylase and α-glucosidase inhibitory activities. The wheat starch digestibility was also reduced by hawthorn flavonoids as well. The results indicated that enzymolysis associated ultrasonic extraction was advisable for extracting flavonoids from hawthorn, and hawthorn flavonoids might be recommended as a potential food supplement with hypoglycemic activities. |
| first_indexed | 2024-04-11T23:52:48Z |
| format | Article |
| id | doaj.art-222babb7252f4539bca05d85cb1b5b67 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T23:52:48Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-222babb7252f4539bca05d85cb1b5b672022-12-22T03:56:26ZengElsevierHeliyon2405-84402022-10-01810e11058Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifidaXin Huang0Yuanyuan Bian1Tianzhi Liu2Zhuang Xu3Ziqi Song4Fei Wang5Tuoping Li6Suhong Li7College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCorresponding author.; College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCorresponding author.; College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaHawthorn flavonoids were extracted by enzymolysis associated ultrasonic procedure. Thirteen flavonoids were identified by HPLC/ESI-QTOF/MS, and the major components were procyanidin C1, rutin-rhamnoside, vitexin-rhamnoside, and catechin. Hawthorn flavonoids exhibited strong free radical scavenging activities against DPPH, ABTS, and hydroxyl radicals. Total and intercellular antioxidant experiments revealed that the free hydro-PSC value was 295.32 ± 12.20 μmol of VCE/g DW, and the free cellular antioxidant activity (CAA) values were 168.60 ± 4.87 μmol of QE/g DW in the no PBS wash protocol and 49.53 ± 1.75 μmol of QE/g DW in the PBS wash protocol. In addition, hawthorn flavonoids exhibited higher α-amylase and α-glucosidase inhibitory activities. The wheat starch digestibility was also reduced by hawthorn flavonoids as well. The results indicated that enzymolysis associated ultrasonic extraction was advisable for extracting flavonoids from hawthorn, and hawthorn flavonoids might be recommended as a potential food supplement with hypoglycemic activities.http://www.sciencedirect.com/science/article/pii/S2405844022023465FlavonoidsAntioxidant activityα-Amylaseα-GlucosidaseStarch digestion |
| spellingShingle | Xin Huang Yuanyuan Bian Tianzhi Liu Zhuang Xu Ziqi Song Fei Wang Tuoping Li Suhong Li Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida Heliyon Flavonoids Antioxidant activity α-Amylase α-Glucosidase Starch digestion |
| title | Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida |
| title_full | Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida |
| title_fullStr | Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida |
| title_full_unstemmed | Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida |
| title_short | Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida |
| title_sort | antioxidant potential and in vitro inhibition of starch digestion of flavonoids from crataegus pinnatifida |
| topic | Flavonoids Antioxidant activity α-Amylase α-Glucosidase Starch digestion |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022023465 |
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