Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human

Summary: Here we describe a multiplex chromogenic immunohistochemistry platform to stain and analyze two markers in paraffin tissue sections from mouse or human. The basis of the protocol is a series of stripping and re-probing steps with subsequent image analysis, which allows the user to perform m...

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Bibliographic Details
Main Authors: Oscar Maiques, Victoria Sanz-Moreno
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007596