Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human
Summary: Here we describe a multiplex chromogenic immunohistochemistry platform to stain and analyze two markers in paraffin tissue sections from mouse or human. The basis of the protocol is a series of stripping and re-probing steps with subsequent image analysis, which allows the user to perform m...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007596 |
| _version_ | 1811292105144795136 |
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| author | Oscar Maiques Victoria Sanz-Moreno |
| author_facet | Oscar Maiques Victoria Sanz-Moreno |
| author_sort | Oscar Maiques |
| collection | DOAJ |
| description | Summary: Here we describe a multiplex chromogenic immunohistochemistry platform to stain and analyze two markers in paraffin tissue sections from mouse or human. The basis of the protocol is a series of stripping and re-probing steps with subsequent image analysis, which allows the user to perform multiplex imaging in a reliable and affordable manner. Here, we describe specific usage to assess the levels of PD-L1 in tumor-associated macrophages. We have used different antibodies and assessed this protocol for up to five consecutive antibodies per slide.For complete details on the use and execution of this protocol, please refer to Orgaz et al. (2020).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T04:40:21Z |
| format | Article |
| id | doaj.art-224cafa47c55421bb3bae9f3d5a08d85 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T04:40:21Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-224cafa47c55421bb3bae9f3d5a08d852022-12-22T03:02:00ZengElsevierSTAR Protocols2666-16672022-12-0134101879Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or humanOscar Maiques0Victoria Sanz-Moreno1Barts Cancer Institute, Queen Mary University of London, John Vane Science Building, Charterhouse Square, London EC1M 6BQ, UKBarts Cancer Institute, Queen Mary University of London, John Vane Science Building, Charterhouse Square, London EC1M 6BQ, UK; Corresponding authorSummary: Here we describe a multiplex chromogenic immunohistochemistry platform to stain and analyze two markers in paraffin tissue sections from mouse or human. The basis of the protocol is a series of stripping and re-probing steps with subsequent image analysis, which allows the user to perform multiplex imaging in a reliable and affordable manner. Here, we describe specific usage to assess the levels of PD-L1 in tumor-associated macrophages. We have used different antibodies and assessed this protocol for up to five consecutive antibodies per slide.For complete details on the use and execution of this protocol, please refer to Orgaz et al. (2020).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007596CancerHigh Throughput ScreeningImmunologyMicroscopyMolecular/Chemical Probes |
| spellingShingle | Oscar Maiques Victoria Sanz-Moreno Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human STAR Protocols Cancer High Throughput Screening Immunology Microscopy Molecular/Chemical Probes |
| title | Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human |
| title_full | Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human |
| title_fullStr | Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human |
| title_full_unstemmed | Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human |
| title_short | Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human |
| title_sort | multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human |
| topic | Cancer High Throughput Screening Immunology Microscopy Molecular/Chemical Probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007596 |
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