Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

Abstract Background Mycobacterium bovis notoriously causes detrimental infections in bovines and humans. In this study, 1500 buffaloes and 2200 cattle were tested by single intradermal comparative cervical tuberculin test and compared with the detection rates of M. bovis isolation, real-time and sim...

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Bibliographic Details
Main Authors: Mohamed Sabry Abd Elraheam Elsayed, Ahmed Salah, Ahmed Abd Elbadee, Tamer Roshdy
Format: Article
Language:English
Published: BMC 2022-06-01
Series:BMC Microbiology
Subjects:
Cattle and buffalo
Conventional PCR
Flow cytometry
Mycobacterium bovis
Real-time PCR atpE
Online Access:https://doi.org/10.1186/s12866-022-02568-0

Internet

https://doi.org/10.1186/s12866-022-02568-0

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