Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering
Abstract Background The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vit...
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-020-02024-8 |
| _version_ | 1818854742509486080 |
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| author | Yuchao Yang Yanting Feng Rongmei Qu Qingtao Li Dongming Rong Tingyu Fan Yiting Yang Bing Sun Zhenyu Bi Asmat Ullah Khan Ting Deng Jingxing Dai Jun Ouyang |
| author_facet | Yuchao Yang Yanting Feng Rongmei Qu Qingtao Li Dongming Rong Tingyu Fan Yiting Yang Bing Sun Zhenyu Bi Asmat Ullah Khan Ting Deng Jingxing Dai Jun Ouyang |
| author_sort | Yuchao Yang |
| collection | DOAJ |
| description | Abstract Background The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and in vivo. Methods To prepare cell culture scaffolds of varying stiffness, increasing amounts of hydroxyapatite (HAp) were mixed into a polyethylene glycol/silk fibroin (PEG/SF) solution. The amount of HAp ranged from 25 to 100 mg, which provided for different ratios between HAp and the PEG/SF composite. In vitro, the effect of stiffness on the osteogenic differentiation of rat BMSCs was studied. The outcome measures, which were verified in vivo, included the protein expression of runt-related transcription factor 2 and osteocalcin, alkaline phosphatase activity, and the mRNA expression of osteogenesis-related markers. Results Increasing amounts of HAp resulted in an increased elastic modulus of the cell culture scaffolds. The PEG/SF/HAp fabricated with HAp (50 mg) significantly increased cell adhesion and viability (p < 0.05) as well as the expression of all the osteogenesis-related markers (p < 0.05). Conclusions We developed a novel cell culture scaffold and demonstrated that substrate stiffness influenced the osteogenic differentiation of rat BMSCs. |
| first_indexed | 2024-12-19T07:57:33Z |
| format | Article |
| id | doaj.art-226bce58e72a49189120ae64da637f14 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-19T07:57:33Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-226bce58e72a49189120ae64da637f142022-12-21T20:29:56ZengBMCStem Cell Research & Therapy1757-65122020-12-0111111710.1186/s13287-020-02024-8Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineeringYuchao Yang0Yanting Feng1Rongmei Qu2Qingtao Li3Dongming Rong4Tingyu Fan5Yiting Yang6Bing Sun7Zhenyu Bi8Asmat Ullah Khan9Ting Deng10Jingxing Dai11Jun Ouyang12Guangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversitySchool of Medicine, South China University of TechnologyDepartment of Orthopedics, Zhujiang Hospital, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics & Department of Anatomy, School of Basic Medical Science, Southern Medical UniversityAbstract Background The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells. In this study, the effect of the biophysical microenvironment on rat bone marrow mesenchymal stem cell (BMSC) osteogenesis was studied both in vitro and in vivo. Methods To prepare cell culture scaffolds of varying stiffness, increasing amounts of hydroxyapatite (HAp) were mixed into a polyethylene glycol/silk fibroin (PEG/SF) solution. The amount of HAp ranged from 25 to 100 mg, which provided for different ratios between HAp and the PEG/SF composite. In vitro, the effect of stiffness on the osteogenic differentiation of rat BMSCs was studied. The outcome measures, which were verified in vivo, included the protein expression of runt-related transcription factor 2 and osteocalcin, alkaline phosphatase activity, and the mRNA expression of osteogenesis-related markers. Results Increasing amounts of HAp resulted in an increased elastic modulus of the cell culture scaffolds. The PEG/SF/HAp fabricated with HAp (50 mg) significantly increased cell adhesion and viability (p < 0.05) as well as the expression of all the osteogenesis-related markers (p < 0.05). Conclusions We developed a novel cell culture scaffold and demonstrated that substrate stiffness influenced the osteogenic differentiation of rat BMSCs.https://doi.org/10.1186/s13287-020-02024-8HydroxyapatiteStiffnessExtracellular matrix (ECM)Bone marrow mesenchymal stem cells (BMSCs)OsteogenesisOsteoinduction |
| spellingShingle | Yuchao Yang Yanting Feng Rongmei Qu Qingtao Li Dongming Rong Tingyu Fan Yiting Yang Bing Sun Zhenyu Bi Asmat Ullah Khan Ting Deng Jingxing Dai Jun Ouyang Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering Stem Cell Research & Therapy Hydroxyapatite Stiffness Extracellular matrix (ECM) Bone marrow mesenchymal stem cells (BMSCs) Osteogenesis Osteoinduction |
| title | Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering |
| title_full | Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering |
| title_fullStr | Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering |
| title_full_unstemmed | Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering |
| title_short | Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering |
| title_sort | synthesis of aligned porous polyethylene glycol silk fibroin hydroxyapatite scaffolds for osteoinduction in bone tissue engineering |
| topic | Hydroxyapatite Stiffness Extracellular matrix (ECM) Bone marrow mesenchymal stem cells (BMSCs) Osteogenesis Osteoinduction |
| url | https://doi.org/10.1186/s13287-020-02024-8 |
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