A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i>
The microalga <i>Porphyridium</i> accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of <i>Porphyridium</i> biomass. In the present study, a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/9/2164 |
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| author | Tao Li Jin Xu Weinan Wang Zishuo Chen Chulin Li Hualian Wu Houbo Wu Wenzhou Xiang |
| author_facet | Tao Li Jin Xu Weinan Wang Zishuo Chen Chulin Li Hualian Wu Houbo Wu Wenzhou Xiang |
| author_sort | Tao Li |
| collection | DOAJ |
| description | The microalga <i>Porphyridium</i> accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of <i>Porphyridium</i> biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.3, 74.3, and 75.2%, respectively. The phycoerythrin exhibited excellent fluorescence characteristics but had low purity. The crude lipid was dark with poor fluidity. Digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol containing C20:5 and C20:4 were the most abundant glycerolipids, while glucose, xylose, and galactose constituted the intracellular polysaccharides that had covalently bound to proteins (8.01%), uronic acid (4.13%), and sulfate (8.31%). Compared with polysaccharides and crude lipids, crude phycoerythrin showed the best antioxidant activity. Overall, the three-step fractional extraction process was feasible for <i>Porphyridium</i>; however, further purification is necessary for downstream applications. |
| first_indexed | 2024-03-10T07:40:20Z |
| format | Article |
| id | doaj.art-2272b482f4964cafb4eebbe993e9c2ba |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T07:40:20Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2272b482f4964cafb4eebbe993e9c2ba2023-11-22T13:05:11ZengMDPI AGFoods2304-81582021-09-01109216410.3390/foods10092164A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i>Tao Li0Jin Xu1Weinan Wang2Zishuo Chen3Chulin Li4Hualian Wu5Houbo Wu6Wenzhou Xiang7CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaThe microalga <i>Porphyridium</i> accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of <i>Porphyridium</i> biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.3, 74.3, and 75.2%, respectively. The phycoerythrin exhibited excellent fluorescence characteristics but had low purity. The crude lipid was dark with poor fluidity. Digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol containing C20:5 and C20:4 were the most abundant glycerolipids, while glucose, xylose, and galactose constituted the intracellular polysaccharides that had covalently bound to proteins (8.01%), uronic acid (4.13%), and sulfate (8.31%). Compared with polysaccharides and crude lipids, crude phycoerythrin showed the best antioxidant activity. Overall, the three-step fractional extraction process was feasible for <i>Porphyridium</i>; however, further purification is necessary for downstream applications.https://www.mdpi.com/2304-8158/10/9/2164<i>Porphyridium purpureum</i>fractional extractionphycoerythrinpolyunsaturated fatty acidpolysaccharides |
| spellingShingle | Tao Li Jin Xu Weinan Wang Zishuo Chen Chulin Li Hualian Wu Houbo Wu Wenzhou Xiang A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i> Foods <i>Porphyridium purpureum</i> fractional extraction phycoerythrin polyunsaturated fatty acid polysaccharides |
| title | A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i> |
| title_full | A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i> |
| title_fullStr | A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i> |
| title_full_unstemmed | A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i> |
| title_short | A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae <i>Porphyridium purpureum</i> |
| title_sort | novel three step extraction strategy for high value products from red algae i porphyridium purpureum i |
| topic | <i>Porphyridium purpureum</i> fractional extraction phycoerythrin polyunsaturated fatty acid polysaccharides |
| url | https://www.mdpi.com/2304-8158/10/9/2164 |
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