Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction
<i>Cynara cardunculus</i> L. inflorescence infusion has been used for several centuries as curd in traditional cheese making, such as some highly prized Portuguese cheeses. To promote the sustainable use of all <i>C. cardunculus</i> plants, <i>C. cardunculus</i> e...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/12/7/3629 |
| _version_ | 1797440249437093888 |
|---|---|
| author | Ana Rita Ferraz Cristina M. B. S. Pintado Maria Luísa Serralheiro |
| author_facet | Ana Rita Ferraz Cristina M. B. S. Pintado Maria Luísa Serralheiro |
| author_sort | Ana Rita Ferraz |
| collection | DOAJ |
| description | <i>Cynara cardunculus</i> L. inflorescence infusion has been used for several centuries as curd in traditional cheese making, such as some highly prized Portuguese cheeses. To promote the sustainable use of all <i>C. cardunculus</i> plants, <i>C. cardunculus</i> extract leaves decoction (CL), inflorescence decoction (CI), chlorogenic acid (CA) (a compound in the plant leaves), and rosmarinic acid (RA) (a similar phenolic compound) solutions were tested for antimicrobial activity against bacteria that may appear on the cheese rind. The antimicrobial activity was evaluated by 15 bacterial strains using two different methodologies: solid and liquid. The influence of these extracts and the phenolic compounds on melanin bioproduction by <i>Pseudomonas putida</i> ESACB 191 was also studied. CA and RA (1 mg/mL) showed antimicrobial activity. CL and CA reduced <i>P. putida</i> ESACB 191 growth in the liquid assay and melanin bioproduction by 6.20 Log CFU/mL and 50%, respectively. Cynarin, CA, and its derivates were identified as the main phenolic compounds (52%) of CL, which may justify its inhibitory action on bacterial growth and melanin bioproduction. Thus, future perspectives include the application of CL extracts with antimicrobial activity in edible films and/or coatings to applied in cheese rind to increase the shelf time. |
| first_indexed | 2024-03-09T12:05:24Z |
| format | Article |
| id | doaj.art-227a45283da04d8cb09994b149f6a9cf |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T12:05:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-227a45283da04d8cb09994b149f6a9cf2023-11-30T22:58:22ZengMDPI AGApplied Sciences2076-34172022-04-01127362910.3390/app12073629Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin BioproductionAna Rita Ferraz0Cristina M. B. S. Pintado1Maria Luísa Serralheiro2Instituto de Biossistemas e Ciências Integrativas (BioISI), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalEscola Superior Agrária, Instituto Politécnico de Castelo Branco, Quinta da Sra. de Mércoles, 6001-909 Castelo Branco, PortugalInstituto de Biossistemas e Ciências Integrativas (BioISI), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal<i>Cynara cardunculus</i> L. inflorescence infusion has been used for several centuries as curd in traditional cheese making, such as some highly prized Portuguese cheeses. To promote the sustainable use of all <i>C. cardunculus</i> plants, <i>C. cardunculus</i> extract leaves decoction (CL), inflorescence decoction (CI), chlorogenic acid (CA) (a compound in the plant leaves), and rosmarinic acid (RA) (a similar phenolic compound) solutions were tested for antimicrobial activity against bacteria that may appear on the cheese rind. The antimicrobial activity was evaluated by 15 bacterial strains using two different methodologies: solid and liquid. The influence of these extracts and the phenolic compounds on melanin bioproduction by <i>Pseudomonas putida</i> ESACB 191 was also studied. CA and RA (1 mg/mL) showed antimicrobial activity. CL and CA reduced <i>P. putida</i> ESACB 191 growth in the liquid assay and melanin bioproduction by 6.20 Log CFU/mL and 50%, respectively. Cynarin, CA, and its derivates were identified as the main phenolic compounds (52%) of CL, which may justify its inhibitory action on bacterial growth and melanin bioproduction. Thus, future perspectives include the application of CL extracts with antimicrobial activity in edible films and/or coatings to applied in cheese rind to increase the shelf time.https://www.mdpi.com/2076-3417/12/7/3629cheese<i>Pseudomonas putida</i><i>C. cardunculus</i>phenolic compoundsantimicrobial activitymelanin bioproduction |
| spellingShingle | Ana Rita Ferraz Cristina M. B. S. Pintado Maria Luísa Serralheiro Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction Applied Sciences cheese <i>Pseudomonas putida</i> <i>C. cardunculus</i> phenolic compounds antimicrobial activity melanin bioproduction |
| title | Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction |
| title_full | Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction |
| title_fullStr | Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction |
| title_full_unstemmed | Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction |
| title_short | Influence of <i>Cynara cardunculus</i> L. Phenolic Compounds on <i>Pseudomonas putida</i> Isolated from the Dairy Industry: Growth and Melanin Bioproduction |
| title_sort | influence of i cynara cardunculus i l phenolic compounds on i pseudomonas putida i isolated from the dairy industry growth and melanin bioproduction |
| topic | cheese <i>Pseudomonas putida</i> <i>C. cardunculus</i> phenolic compounds antimicrobial activity melanin bioproduction |
| url | https://www.mdpi.com/2076-3417/12/7/3629 |
| work_keys_str_mv | AT anaritaferraz influenceoficynaracardunculusilphenoliccompoundsonipseudomonasputidaiisolatedfromthedairyindustrygrowthandmelaninbioproduction AT cristinambspintado influenceoficynaracardunculusilphenoliccompoundsonipseudomonasputidaiisolatedfromthedairyindustrygrowthandmelaninbioproduction AT marialuisaserralheiro influenceoficynaracardunculusilphenoliccompoundsonipseudomonasputidaiisolatedfromthedairyindustrygrowthandmelaninbioproduction |