Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes

Abstract Background The CRISPR/Cas9 is currently the predominant technology to enhance the genome editing efficiency in eukaryotes. Established tools for many fungal species exist while most of them are based on in vivo expressed Cas9 and guide RNA (gRNA). Alternatively, in vitro assembled Cas9 and...

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Bibliographic Details
Main Authors: Joosu Kuivanen, Veera Korja, Sami Holmström, Peter Richard
Format: Article
Language:English
Published: BMC 2019-03-01
Series:Fungal Biology and Biotechnology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s40694-019-0066-9