Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes
Abstract Background The CRISPR/Cas9 is currently the predominant technology to enhance the genome editing efficiency in eukaryotes. Established tools for many fungal species exist while most of them are based on in vivo expressed Cas9 and guide RNA (gRNA). Alternatively, in vitro assembled Cas9 and...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2019-03-01
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Series: | Fungal Biology and Biotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s40694-019-0066-9 |