PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway
Abstract Background Phosphoglucomutase 1 (PGM1) is known for its involvement in cancer pathogenesis. However, its biological role in colorectal cancer (CRC) has remained unknown. Here, we studied the functions and mechanisms of PGM1 in CRC. Methods We verified PGM-1 as a differentially expressed gen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-022-02545-7 |
| _version_ | 1818213417406693376 |
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| author | Zhewen Zheng Xue Zhang Jian Bai Long Long Di Liu Yunfeng Zhou |
| author_facet | Zhewen Zheng Xue Zhang Jian Bai Long Long Di Liu Yunfeng Zhou |
| author_sort | Zhewen Zheng |
| collection | DOAJ |
| description | Abstract Background Phosphoglucomutase 1 (PGM1) is known for its involvement in cancer pathogenesis. However, its biological role in colorectal cancer (CRC) has remained unknown. Here, we studied the functions and mechanisms of PGM1 in CRC. Methods We verified PGM-1 as a differentially expressed gene (DEG) by employing a comprehensive strategy of TCGA-COAD dataset mining and computational biology. Relative levels of PGM-1 in CRC tumors and adjoining peritumoral tissues were determined by qRT-PCR, western blotting (WB), and immunohistochemical (IHC) staining in a tissue microarray. PGM1 functions were analyzed by CCK8, EdU, colony formation, cell cycle, apoptosis, and Transwell migration and invasion assays. The influence of PGM1 was further investigated by studying tumor formation in vivo. Results The levels of PGM1 mRNA and protein were both reduced in CRC tissues, and the reductions were related to CRC pathology and overall survival. PGM1 knockdown stimulated both cell proliferation and colony formation, and inhibited cell cycle arrest and apoptosis, while overexpression of PGM1 produced the opposite effects in CRC cells both in vivo and in vitro. Furthermore, the effects of PGM1 were related to the PI3K/ AKT pathway. Conclusion We verified that PGM1 suppresses CRC progression via the PI3K/AKT pathway. These results suggest the potential for targeting PGM1 in treatment of CRC. |
| first_indexed | 2024-12-12T06:03:57Z |
| format | Article |
| id | doaj.art-228472390e8e456aafb2b3dd0370c573 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-12T06:03:57Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-228472390e8e456aafb2b3dd0370c5732022-12-22T00:35:19ZengBMCCancer Cell International1475-28672022-05-0122111810.1186/s12935-022-02545-7PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathwayZhewen Zheng0Xue Zhang1Jian Bai2Long Long3Di Liu4Yunfeng Zhou5Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital of Wuhan UniversityDepartment of General Practice, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Anesthesiology, Peking University Third HospitalDepartment of Radiation Oncology and Medical Oncology, Zhongnan Hospital of Wuhan UniversityDepartment of Radiation Oncology and Medical Oncology, Zhongnan Hospital of Wuhan UniversityDepartment of Radiation Oncology and Medical Oncology, Zhongnan Hospital of Wuhan UniversityAbstract Background Phosphoglucomutase 1 (PGM1) is known for its involvement in cancer pathogenesis. However, its biological role in colorectal cancer (CRC) has remained unknown. Here, we studied the functions and mechanisms of PGM1 in CRC. Methods We verified PGM-1 as a differentially expressed gene (DEG) by employing a comprehensive strategy of TCGA-COAD dataset mining and computational biology. Relative levels of PGM-1 in CRC tumors and adjoining peritumoral tissues were determined by qRT-PCR, western blotting (WB), and immunohistochemical (IHC) staining in a tissue microarray. PGM1 functions were analyzed by CCK8, EdU, colony formation, cell cycle, apoptosis, and Transwell migration and invasion assays. The influence of PGM1 was further investigated by studying tumor formation in vivo. Results The levels of PGM1 mRNA and protein were both reduced in CRC tissues, and the reductions were related to CRC pathology and overall survival. PGM1 knockdown stimulated both cell proliferation and colony formation, and inhibited cell cycle arrest and apoptosis, while overexpression of PGM1 produced the opposite effects in CRC cells both in vivo and in vitro. Furthermore, the effects of PGM1 were related to the PI3K/ AKT pathway. Conclusion We verified that PGM1 suppresses CRC progression via the PI3K/AKT pathway. These results suggest the potential for targeting PGM1 in treatment of CRC.https://doi.org/10.1186/s12935-022-02545-7Phosphoglucomutase 1Colorectal cancerPrognosisProliferationApoptosis |
| spellingShingle | Zhewen Zheng Xue Zhang Jian Bai Long Long Di Liu Yunfeng Zhou PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway Cancer Cell International Phosphoglucomutase 1 Colorectal cancer Prognosis Proliferation Apoptosis |
| title | PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway |
| title_full | PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway |
| title_fullStr | PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway |
| title_full_unstemmed | PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway |
| title_short | PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway |
| title_sort | pgm1 suppresses colorectal cancer cell migration and invasion by regulating the pi3k akt pathway |
| topic | Phosphoglucomutase 1 Colorectal cancer Prognosis Proliferation Apoptosis |
| url | https://doi.org/10.1186/s12935-022-02545-7 |
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