Protein engineering of NADH pyrophosphatase for efficient biocatalytic production of reduced nicotinamide mononucleotide

Protein engineering of NADH pyrophosphatase for efficient biocatalytic production of reduced nicotinamide mononucleotide

Introduction: NADH pyrophosphatase, a hydrolase catalyzing the phosphate bond of NADH to reduced nicotinamide mononucleotide, has potential applications in the food, cosmetic and pharmaceutical industry.Methods: Here, we investigated the effects of vector screening, promoter and RBS strategies on NA...

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Bibliographic Details
Main Authors: Ye Liu, Jin-Song Gong, George Marshall, Chang Su, Michael Hall, Heng Li, Guo-Qiang Xu, Jin-Song Shi, Zheng-Hong Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
reduced nicotinamide mononucleotide
NADH pyrophosphatase
expression
semi-rational engineering
protein engineering
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1159965/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2023.1159965/full

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