Examination of the Catalytic Role of the Axial Cystine Ligand in the Co-Type Nitrile Hydratase from <i>Pseudonocardia thermophila</i> JCM 3095
The strictly conserved αSer162 residue in the Co-type nitrile hydratase from <i>Pseudonocardia thermophila</i> JCM 3095 (<i>Pt</i>NHase), which forms a hydrogen bond to the axial αCys108-S atom, was mutated into an Ala residue. The αSer162Ala yielded two different protein spe...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-11-01
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Series: | Catalysts |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4344/11/11/1381 |