Examination of the Catalytic Role of the Axial Cystine Ligand in the Co-Type Nitrile Hydratase from <i>Pseudonocardia thermophila</i> JCM 3095

Examination of the Catalytic Role of the Axial Cystine Ligand in the Co-Type Nitrile Hydratase from <i>Pseudonocardia thermophila</i> JCM 3095

The strictly conserved αSer162 residue in the Co-type nitrile hydratase from <i>Pseudonocardia thermophila</i> JCM 3095 (<i>Pt</i>NHase), which forms a hydrogen bond to the axial αCys108-S atom, was mutated into an Ala residue. The αSer162Ala yielded two different protein spe...

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Bibliographic Details
Main Authors: Irene R. A. M. Ogutu, Martin St. Maurice, Brian Bennett, Richard C. Holz
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Catalysts
Subjects:
nitrile hydratase
cobalt
hydration
mutant
X-ray crystallography
UV–vis spectroscopy
Online Access:https://www.mdpi.com/2073-4344/11/11/1381

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