Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells

Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells

Dopamine receptors are G-protein-coupled receptors that are connected to severe neurological disorders. The development of new ligands targeting these receptors enables gaining a deeper insight into the receptor functioning, including binding mechanisms, kinetics and oligomerization. Novel fluoresce...

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Bibliographic Details
Main Authors: Maris-Johanna Tahk, Tõnis Laasfeld, Elo Meriste, Jose Brea, Maria Isabel Loza, Maria Majellaro, Marialessandra Contino, Eddy Sotelo, Ago Rinken
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Molecular Biosciences
Subjects:
G protein-coupled receptor (GPCR)
dopamine D3 receptor (D3R)
ligand binding kinetics
fluorescent probe
deep-learning based image analysis
budded baculoviruses
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2023.1119157/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2023.1119157/full

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