Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis
IntroductionWe aimed to identify B-cell-mediated immunomechanisms in inclusion body myositis (IBM) and polymyositis (PM) as part of the complex pathophysiology.Materials and methodsHuman primary myotube cultures were derived from orthopedic surgery. Diagnostic biopsy specimens from patients with IBM...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1177721/full |
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| author | Per-Ole Carstens Luisa M. Müllar Arne Wrede Arne Wrede Sabrina Zechel Martin M. Wachowski Almuth Brandis Almuth Brandis Sabine Krause Stephan Zierz Jens Schmidt Jens Schmidt Jens Schmidt |
| author_facet | Per-Ole Carstens Luisa M. Müllar Arne Wrede Arne Wrede Sabrina Zechel Martin M. Wachowski Almuth Brandis Almuth Brandis Sabine Krause Stephan Zierz Jens Schmidt Jens Schmidt Jens Schmidt |
| author_sort | Per-Ole Carstens |
| collection | DOAJ |
| description | IntroductionWe aimed to identify B-cell-mediated immunomechanisms in inclusion body myositis (IBM) and polymyositis (PM) as part of the complex pathophysiology.Materials and methodsHuman primary myotube cultures were derived from orthopedic surgery. Diagnostic biopsy specimens from patients with IBM (n=9) and PM (n=9) were analyzed for markers of B cell activation (BAFF and APRIL) and for chemokines that control the recruitment of B cells (CXCL-12 and CXCL-13). Results were compared to biopsy specimens without myopathic changes (n=9) and hereditary muscular dystrophy (n=9).ResultsThe mRNA expression of BAFF, APRIL, and CXCL-13 was significantly higher in IBM and PM compared to controls. Patients with IBM displayed the highest number of double positive muscle fibers for BAFF and CXCL-12 (48%) compared to PM (25%), muscular dystrophy (3%), and non-myopathic controls (0%). In vitro, exposure of human myotubes to pro-inflammatory cytokines led to a significant upregulation of BAFF and CXCL-12, but APRIL and CXCL-13 remained unchanged.ConclusionThe results substantiate the hypothesis of an involvement of B cell-associated mechanisms in the pathophysiology of IBM and PM. Muscle fibers themselves seem to contribute to the recruitment of B cells and sustain inflammation. |
| first_indexed | 2024-03-12T02:28:42Z |
| format | Article |
| id | doaj.art-23060bdf8e2743068f853639ee4aa7c1 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T02:28:42Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-23060bdf8e2743068f853639ee4aa7c12023-09-05T09:27:54ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-09-011410.3389/fimmu.2023.11777211177721Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositisPer-Ole Carstens0Luisa M. Müllar1Arne Wrede2Arne Wrede3Sabrina Zechel4Martin M. Wachowski5Almuth Brandis6Almuth Brandis7Sabine Krause8Stephan Zierz9Jens Schmidt10Jens Schmidt11Jens Schmidt12Department of Neurology, University Medical Center Göttingen, Göttingen, GermanyDepartment of Neurology, University Medical Center Göttingen, Göttingen, GermanyInstitute of Neuropathology, University Medical Center Göttingen, Göttingen, GermanyInstitute of Neuropathology, Saarland University Medical Center and Medical Faculty of Saarland University, Homburg, GermanyInstitute of Neuropathology, University Medical Center Göttingen, Göttingen, GermanyDepartment of Trauma Surgery, Orthopaedics and Plastic Surgery, University Medical Center Göttingen, Göttingen, GermanyDepartment of Pathology, Klinikum Region Hannover, Hannover, GermanyInstitute of Pathology and Neuropathology, Medical University Hannover, Hannover, GermanyFriedrich-Baur-Institute, Department of Neurology, Ludwig-Maximilians-University of München, München, GermanyDepartment of Neurology, University Hospital Halle/Saale, Halle, GermanyDepartment of Neurology, University Medical Center Göttingen, Göttingen, GermanyDepartment of Neurology and Pain Treatment, Neuromuscular Center, Center for Translational Medicine, Immanuel Klinik Rüdersdorf, University Hospital of the Brandenburg Medical School Theodor Fontane, Rüdersdorf bei Berlin, Germany0Faculty of Health Sciences Brandenburg, Brandenburg Medical School Theodor Fontane, Rüdersdorf bei, Berlin, GermanyIntroductionWe aimed to identify B-cell-mediated immunomechanisms in inclusion body myositis (IBM) and polymyositis (PM) as part of the complex pathophysiology.Materials and methodsHuman primary myotube cultures were derived from orthopedic surgery. Diagnostic biopsy specimens from patients with IBM (n=9) and PM (n=9) were analyzed for markers of B cell activation (BAFF and APRIL) and for chemokines that control the recruitment of B cells (CXCL-12 and CXCL-13). Results were compared to biopsy specimens without myopathic changes (n=9) and hereditary muscular dystrophy (n=9).ResultsThe mRNA expression of BAFF, APRIL, and CXCL-13 was significantly higher in IBM and PM compared to controls. Patients with IBM displayed the highest number of double positive muscle fibers for BAFF and CXCL-12 (48%) compared to PM (25%), muscular dystrophy (3%), and non-myopathic controls (0%). In vitro, exposure of human myotubes to pro-inflammatory cytokines led to a significant upregulation of BAFF and CXCL-12, but APRIL and CXCL-13 remained unchanged.ConclusionThe results substantiate the hypothesis of an involvement of B cell-associated mechanisms in the pathophysiology of IBM and PM. Muscle fibers themselves seem to contribute to the recruitment of B cells and sustain inflammation.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1177721/fullautoimmune diseasesneuromuscular diseaseinflammatory muscle diseasemyositisB cells |
| spellingShingle | Per-Ole Carstens Luisa M. Müllar Arne Wrede Arne Wrede Sabrina Zechel Martin M. Wachowski Almuth Brandis Almuth Brandis Sabine Krause Stephan Zierz Jens Schmidt Jens Schmidt Jens Schmidt Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis Frontiers in Immunology autoimmune diseases neuromuscular disease inflammatory muscle disease myositis B cells |
| title | Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis |
| title_full | Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis |
| title_fullStr | Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis |
| title_full_unstemmed | Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis |
| title_short | Skeletal muscle fibers produce B-cell stimulatory factors in chronic myositis |
| title_sort | skeletal muscle fibers produce b cell stimulatory factors in chronic myositis |
| topic | autoimmune diseases neuromuscular disease inflammatory muscle disease myositis B cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1177721/full |
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