Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus)
In this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish（Sciaenops ocellatus ）scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chro...
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The editorial department of Science and Technology of Food Industry
2022-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060150 |
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| author | Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU |
| author_facet | Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU |
| author_sort | Jingliang WAN |
| collection | DOAJ |
| description | In this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish（Sciaenops ocellatus ）scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chromatography. Polypeptide metal chelates were synthesized by water system method and the metal chelating rate was determined by EDTA complexometric titration method. L9(34) orthogonal test was used to optimize the processing conditions of polypeptide metal chelates. The binding of polypeptide to metal was verified by detecting polypeptide and metal chelate by infrared spectroscopy. The molecular weight of polypeptide was about 1~6 kDa by gel chromatography analysis. The preparation conditions of polypeptide zinc chelate were obtained by orthogonal experiment, that is, the mass ratio of zinc chelate was 2:1, pH5.5, temperature 55℃, time 60 min. The optimal chelating conditions were mass ratio 2:1, pH6.0, temperature 35℃ and time 55 min. Under the optimized condition, the chelate ratio of zinc and copper were 78.21% and 91.24% respectively. The infrared absorption spectra showed that Zn2+, Cu2+ and -NH2 were successfully combined. The results showed that the chelation rate was significantly improved under the condition of this process, and it was rich in metal ions, low cost and promising market prospect, which would be conducive to the high-value application of marine fishery processing by-products and promote the technological upgrading of fishery processing industry. |
| first_indexed | 2024-04-11T06:52:05Z |
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| institution | Directory Open Access Journal |
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| language | zho |
| last_indexed | 2024-04-11T06:52:05Z |
| publishDate | 2022-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-2309501142424eb7ae2ca774ddf831192022-12-22T04:39:08ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-04-0143816517110.13386/j.issn1002-0306.20210601502021060150-8Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus)Jingliang WAN0Peng WU1Ling LIN2Yuansen LIU3Xu TANG4Chang’an XU5Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaIn this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish（Sciaenops ocellatus ）scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chromatography. Polypeptide metal chelates were synthesized by water system method and the metal chelating rate was determined by EDTA complexometric titration method. L9(34) orthogonal test was used to optimize the processing conditions of polypeptide metal chelates. The binding of polypeptide to metal was verified by detecting polypeptide and metal chelate by infrared spectroscopy. The molecular weight of polypeptide was about 1~6 kDa by gel chromatography analysis. The preparation conditions of polypeptide zinc chelate were obtained by orthogonal experiment, that is, the mass ratio of zinc chelate was 2:1, pH5.5, temperature 55℃, time 60 min. The optimal chelating conditions were mass ratio 2:1, pH6.0, temperature 35℃ and time 55 min. Under the optimized condition, the chelate ratio of zinc and copper were 78.21% and 91.24% respectively. The infrared absorption spectra showed that Zn2+, Cu2+ and -NH2 were successfully combined. The results showed that the chelation rate was significantly improved under the condition of this process, and it was rich in metal ions, low cost and promising market prospect, which would be conducive to the high-value application of marine fishery processing by-products and promote the technological upgrading of fishery processing industry.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060150fish scales; sciaenops ocellatuspolypeptidepolypeptide metal chelateprocess optimization |
| spellingShingle | Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) Shipin gongye ke-ji fish scales; sciaenops ocellatus polypeptide polypeptide metal chelate process optimization |
| title | Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
| title_full | Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
| title_fullStr | Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
| title_full_unstemmed | Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
| title_short | Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
| title_sort | optimization of preparation process of polypeptide metal chelates from the scales of american red fish sciaenops ocellatus |
| topic | fish scales; sciaenops ocellatus polypeptide polypeptide metal chelate process optimization |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060150 |
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