Lipofection of Non-integrative CRISPR/Cas9 Ribonucleoproteins in Male Germline Stem Cells: A Simple and Effective Knockout Tool for Germline Genome Engineering

Lipofection of Non-integrative CRISPR/Cas9 Ribonucleoproteins in Male Germline Stem Cells: A Simple and Effective Knockout Tool for Germline Genome Engineering

Gene editing in male germline stem (GS) cells is a potent tool to study spermatogenesis and to create transgenic mice. Various engineered nucleases already demonstrated the ability to modify the genome of GS cells. However, current systems are limited by technical complexity diminishing application...

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Bibliographic Details
Main Authors: Mariella Obermeier, Jim Vadolas, Stefaan Verhulst, Ellen Goossens, Yoni Baert
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
gene editing
CRISPR/Cas
germline stem cells
ribonucleoproteins (RNPs)
germline genome editing
gene knockout
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2022.891173/full

Internet

https://www.frontiersin.org/articles/10.3389/fcell.2022.891173/full

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