A Quantitative Fluorescence-Based Lipase Assay

An easy and fast gel diffusion assay for detecting and monitoring lipase activity by quantification of fluorescein is described. By measuring the intensity of fluorescein, it is possible to obtain a calibration curve with a regression coefficient better than by using the radius of fluorescent haloes...

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Bibliographic Details
Main Authors: Giovanna Lomolino, Giovanna Di Pierro, Anna Lante
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2012-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/139103