Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro
Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru (C. jamacaru) (mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collec...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=2;spage=66;epage=72;aulast=Dutra |
| _version_ | 1828783531231281152 |
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| author | Jean Carlos Vencioneck Dutra Judá Ben-Hur de Oliveira Vanessa Silva dos Santos Paula Roberta Costalonga Pereira Jean Moisés Ferreira Maria do Carmo Pimentel Batitucci |
| author_facet | Jean Carlos Vencioneck Dutra Judá Ben-Hur de Oliveira Vanessa Silva dos Santos Paula Roberta Costalonga Pereira Jean Moisés Ferreira Maria do Carmo Pimentel Batitucci |
| author_sort | Jean Carlos Vencioneck Dutra |
| collection | DOAJ |
| description | Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru (C. jamacaru) (mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe2+ ion chelating activity, and in vitro antiproliferative effects (sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe2+ ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent. |
| first_indexed | 2024-12-11T23:13:44Z |
| format | Article |
| id | doaj.art-236220b60b7c48b7ba7051d27926925f |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-11T23:13:44Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-236220b60b7c48b7ba7051d27926925f2022-12-22T00:46:36ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912019-01-0192667210.4103/2221-1691.250857Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitroJean Carlos Vencioneck DutraJudá Ben-Hur de OliveiraVanessa Silva dos SantosPaula Roberta Costalonga PereiraJean Moisés FerreiraMaria do Carmo Pimentel BatitucciObjective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru (C. jamacaru) (mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe2+ ion chelating activity, and in vitro antiproliferative effects (sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe2+ ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=2;spage=66;epage=72;aulast=Dutramandacaruflavonoid contentdpphabtsfe2+ ion chelating activitymtt assaysarcoma 180 cells |
| spellingShingle | Jean Carlos Vencioneck Dutra Judá Ben-Hur de Oliveira Vanessa Silva dos Santos Paula Roberta Costalonga Pereira Jean Moisés Ferreira Maria do Carmo Pimentel Batitucci Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro Asian Pacific Journal of Tropical Biomedicine mandacaru flavonoid content dpph abts fe2+ ion chelating activity mtt assay sarcoma 180 cells |
| title | Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro |
| title_full | Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro |
| title_fullStr | Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro |
| title_full_unstemmed | Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro |
| title_short | Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro |
| title_sort | fruiting increases total content of flavonoids and antiproliferative effects of cereus jamacaru d c cladodes in sarcoma 180 cells in vitro |
| topic | mandacaru flavonoid content dpph abts fe2+ ion chelating activity mtt assay sarcoma 180 cells |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=2;spage=66;epage=72;aulast=Dutra |
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