CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

Abstract Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase...

Full description

Bibliographic Details
Main Authors: Derek H. Janssens, Dominik J. Otto, Michael P. Meers, Manu Setty, Kami Ahmad, Steven Henikoff
Format: Article
Language:English
Published: BMC 2022-03-01
Series:Genome Biology
Subjects:
Single-cell genomics
Chromatin profiling
Epigenomic signal deconvolution
Multi-omics
Online Access:https://doi.org/10.1186/s13059-022-02642-w

Internet

https://doi.org/10.1186/s13059-022-02642-w

Similar Items