Simultaneous Editing of Two Copies of Gh14-3-3d Confers Enhanced Transgene-Clean Plant Defense Against Verticillium dahliae in Allotetraploid Upland Cotton

Simultaneous Editing of Two Copies of Gh14-3-3d Confers Enhanced Transgene-Clean Plant Defense Against Verticillium dahliae in Allotetraploid Upland Cotton

Gossypium hirsutum is an allotetraploid species, meaning that mutants that are difficult to be generated by classical approaches due to gene redundancy. The CRISPR/Cas9 genome editing system is a robust and highly efficient tool for generating target gene mutants, by which the genes of interest may...

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Bibliographic Details
Main Authors: Zhennan Zhang, Xiaoyang Ge, Xiaoli Luo, Peng Wang, Qiang Fan, Guang Hu, Juanli Xiao, Fuguang Li, Jiahe Wu
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Plant Science
Subjects:
Gossypium hirsutum L.
allotetraploid cotton
simultaneous editing
transgene-clean
CRISPR/Cas9
Verticillium dahliae
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.00842/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2018.00842/full

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