Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy
Objective To observe the effect of pregabalin on the expression of Bcl⁃2 and Bax in hippocampus of chronic epileptic rats induced by pilocarpine, to explore the anti⁃epileptic pharmacology mechanism of pregabalin, and its anti ⁃ apoptotic effect on hippocampal neurons of rats. Methods The model of c...
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| Format: | Article |
| Language: | English |
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Tianjin Huanhu Hospital
2012-04-01
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| Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
| Subjects: | |
| Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/147 |
| _version_ | 1811308431272837120 |
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| author | Yi⁃dan ZHANG Shi⁃jing WU Hua⁃ying TAO Xiu⁃na ZHANG |
| author_facet | Yi⁃dan ZHANG Shi⁃jing WU Hua⁃ying TAO Xiu⁃na ZHANG |
| author_sort | Yi⁃dan ZHANG |
| collection | DOAJ |
| description | Objective To observe the effect of pregabalin on the expression of Bcl⁃2 and Bax in hippocampus of chronic epileptic rats induced by pilocarpine, to explore the anti⁃epileptic pharmacology mechanism of pregabalin, and its anti ⁃ apoptotic effect on hippocampal neurons of rats. Methods The model of chronic temporal lobe epileptic rats induced by lithium⁃pilocarpine was established, then the rats in pregabalin treatment group received intraperitoneal injection of pregabalin (40 mg/kg) once daily for three weeks. The expression of Bcl⁃2 and Bax in hippocampus of all rats was detected by immunohistochemical technique and Western blotting. Results Compared with normal saline group rats, the expression of Bcl⁃2 and Bax in hippocampus of rats with chronic temporal lobe epilepsy was significantly increased (P = 0.000, for all). Pregabalin can down⁃regulate the expression of Bax and up⁃regulate the expression of Bcl⁃2 in hippocampus of rats compared to model group rats (P = 0.000, for all). Conclusion Pregabalin may have the effects of inhibiting cell apoptosis and protecting neurons through lowing Bax level and increasing Bcl⁃2 level in hippocampus of chronic temporal lobe epileptic rats.
DOI：10.3969/j.issn.1672⁃6731.2012.02.018 |
| first_indexed | 2024-04-13T09:22:56Z |
| format | Article |
| id | doaj.art-242c4fb8929744bc90b18c2a29b01f43 |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-04-13T09:22:56Z |
| publishDate | 2012-04-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-242c4fb8929744bc90b18c2a29b01f432022-12-22T02:52:31ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312012-04-01122184189146Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsyYi⁃dan ZHANGShi⁃jing WUHua⁃ying TAOXiu⁃na ZHANGObjective To observe the effect of pregabalin on the expression of Bcl⁃2 and Bax in hippocampus of chronic epileptic rats induced by pilocarpine, to explore the anti⁃epileptic pharmacology mechanism of pregabalin, and its anti ⁃ apoptotic effect on hippocampal neurons of rats. Methods The model of chronic temporal lobe epileptic rats induced by lithium⁃pilocarpine was established, then the rats in pregabalin treatment group received intraperitoneal injection of pregabalin (40 mg/kg) once daily for three weeks. The expression of Bcl⁃2 and Bax in hippocampus of all rats was detected by immunohistochemical technique and Western blotting. Results Compared with normal saline group rats, the expression of Bcl⁃2 and Bax in hippocampus of rats with chronic temporal lobe epilepsy was significantly increased (P = 0.000, for all). Pregabalin can down⁃regulate the expression of Bax and up⁃regulate the expression of Bcl⁃2 in hippocampus of rats compared to model group rats (P = 0.000, for all). Conclusion Pregabalin may have the effects of inhibiting cell apoptosis and protecting neurons through lowing Bax level and increasing Bcl⁃2 level in hippocampus of chronic temporal lobe epileptic rats. DOI：10.3969/j.issn.1672⁃6731.2012.02.018http://www.cjcnn.org/index.php/cjcnn/article/view/147Epilepsy, temporal lobeApoptosisGenes, bcl⁃2bcl⁃2⁃associated X proteinAnticonvulsantsImmunohistochemistryBlotting, western |
| spellingShingle | Yi⁃dan ZHANG Shi⁃jing WU Hua⁃ying TAO Xiu⁃na ZHANG Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy Chinese Journal of Contemporary Neurology and Neurosurgery Epilepsy, temporal lobe Apoptosis Genes, bcl⁃2 bcl⁃2⁃associated X protein Anticonvulsants Immunohistochemistry Blotting, western |
| title | Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy |
| title_full | Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy |
| title_fullStr | Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy |
| title_full_unstemmed | Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy |
| title_short | Effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy |
| title_sort | effect of pregabalin on apoptotic regulatory genes in hippocampus of rats with chronic temporal lobe epilepsy |
| topic | Epilepsy, temporal lobe Apoptosis Genes, bcl⁃2 bcl⁃2⁃associated X protein Anticonvulsants Immunohistochemistry Blotting, western |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/147 |
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