Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from <i>Ruminococcus bromii</i>

Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from <i>Ruminococcus bromii</i>

Type V Cas12a nucleases are DNA editors working in a wide temperature range and using expanded protospacer-adjacent motifs (PAMs). Though they are widely used, there is still a demand for discovering new ones. Here, we demonstrate a novel ortholog from <i>Ruminococcus bromii</i> sp. enti...

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Bibliographic Details
Main Authors: Ruslan Vasilev, Natalia Gunitseva, Regina Shebanova, Aleksei Korzhenkov, Anna Vlaskina, Marta Evteeva, Irina Polushkina, Natalia Nikitchina, Stepan Toshchakov, Piotr Kamenski, Maxim Patrushev, Ilya Mazunin
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:International Journal of Molecular Sciences
Subjects:
Cas endonuclease
CRISPR
genome editing
mammalian cells
site-directed mutagenesis
Online Access:https://www.mdpi.com/1422-0067/23/16/9289

Internet

https://www.mdpi.com/1422-0067/23/16/9289

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