Direct comparison of distinct naive pluripotent states in human embryonic stem cells
Human embryonic stem cells (hESCs) in culture display a state of primed pluripotency, but recent protocols have been developed that enable hESCs to adopt a naive-like pluripotent state. Here the authors perform a side-by-side comparison of methods used to culture naive hESCs and confirm the role of...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms15055 |
| _version_ | 1818857848096948224 |
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| author | S. Warrier M. Van der Jeught G. Duggal L. Tilleman E. Sutherland J. Taelman M. Popovic S. Lierman S. Chuva De Sousa Lopes A. Van Soom L. Peelman F. Van Nieuwerburgh D. I. M. De Coninck B. Menten P. Mestdagh J. Van de Sompele D. Deforce P. De Sutter B. Heindryckx |
| author_facet | S. Warrier M. Van der Jeught G. Duggal L. Tilleman E. Sutherland J. Taelman M. Popovic S. Lierman S. Chuva De Sousa Lopes A. Van Soom L. Peelman F. Van Nieuwerburgh D. I. M. De Coninck B. Menten P. Mestdagh J. Van de Sompele D. Deforce P. De Sutter B. Heindryckx |
| author_sort | S. Warrier |
| collection | DOAJ |
| description | Human embryonic stem cells (hESCs) in culture display a state of primed pluripotency, but recent protocols have been developed that enable hESCs to adopt a naive-like pluripotent state. Here the authors perform a side-by-side comparison of methods used to culture naive hESCs and confirm the role of PI3K/AKT/mTORC signalling in facilitating the induction of naive pluripotency. |
| first_indexed | 2024-12-19T08:46:54Z |
| format | Article |
| id | doaj.art-244cbf649add464c82660353beddd982 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T08:46:54Z |
| publishDate | 2017-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-244cbf649add464c82660353beddd9822022-12-21T20:28:48ZengNature PortfolioNature Communications2041-17232017-04-018111010.1038/ncomms15055Direct comparison of distinct naive pluripotent states in human embryonic stem cellsS. Warrier0M. Van der Jeught1G. Duggal2L. Tilleman3E. Sutherland4J. Taelman5M. Popovic6S. Lierman7S. Chuva De Sousa Lopes8A. Van Soom9L. Peelman10F. Van Nieuwerburgh11D. I. M. De Coninck12B. Menten13P. Mestdagh14J. Van de Sompele15D. Deforce16P. De Sutter17B. Heindryckx18Department for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityDepartment of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityDepartment of Pediatrics and Medical Genetics, Center for Medical Genetics, Ghent University HospitalDepartment of Pediatrics and Medical Genetics, Center for Medical Genetics, Ghent University HospitalDepartment of Pediatrics and Medical Genetics, Center for Medical Genetics, Ghent University HospitalDepartment of Pharmaceutics, Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent UniversityDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalDepartment for Reproductive Medicine, Ghent-Fertility and Stem cell Team (G-FaST), Ghent University HospitalHuman embryonic stem cells (hESCs) in culture display a state of primed pluripotency, but recent protocols have been developed that enable hESCs to adopt a naive-like pluripotent state. Here the authors perform a side-by-side comparison of methods used to culture naive hESCs and confirm the role of PI3K/AKT/mTORC signalling in facilitating the induction of naive pluripotency.https://doi.org/10.1038/ncomms15055 |
| spellingShingle | S. Warrier M. Van der Jeught G. Duggal L. Tilleman E. Sutherland J. Taelman M. Popovic S. Lierman S. Chuva De Sousa Lopes A. Van Soom L. Peelman F. Van Nieuwerburgh D. I. M. De Coninck B. Menten P. Mestdagh J. Van de Sompele D. Deforce P. De Sutter B. Heindryckx Direct comparison of distinct naive pluripotent states in human embryonic stem cells Nature Communications |
| title | Direct comparison of distinct naive pluripotent states in human embryonic stem cells |
| title_full | Direct comparison of distinct naive pluripotent states in human embryonic stem cells |
| title_fullStr | Direct comparison of distinct naive pluripotent states in human embryonic stem cells |
| title_full_unstemmed | Direct comparison of distinct naive pluripotent states in human embryonic stem cells |
| title_short | Direct comparison of distinct naive pluripotent states in human embryonic stem cells |
| title_sort | direct comparison of distinct naive pluripotent states in human embryonic stem cells |
| url | https://doi.org/10.1038/ncomms15055 |
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