Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression
Diet plays a substantial role in the pathogenesis and management of ulcerative colitis (UC), and epidemiologic studies indicate an association between red meat intake and increased risk of UC development. Therefore, we evaluated the effect of a red meat diet on dextran sulfate sodium (DSS)-induced c...
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MDPI AG
2020-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/6/1728 |
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| author | Tina S. Nielsen Marlene Fredborg Peter K. Theil Yuan Yue Lærke V. Bruhn Vibeke Andersen Stig Purup |
| author_facet | Tina S. Nielsen Marlene Fredborg Peter K. Theil Yuan Yue Lærke V. Bruhn Vibeke Andersen Stig Purup |
| author_sort | Tina S. Nielsen |
| collection | DOAJ |
| description | Diet plays a substantial role in the pathogenesis and management of ulcerative colitis (UC), and epidemiologic studies indicate an association between red meat intake and increased risk of UC development. Therefore, we evaluated the effect of a red meat diet on dextran sulfate sodium (DSS)-induced colitis in pigs. Weaned pigs (42 days old) were fed either a control diet or a diet substituted with 15% minced, cooked and dried beef from experimental day 0 to 14. From day 14 to 18, half of the pigs on each diet received a daily oral dose of DSS. Dietary red meat aggravated the severity of colitis based on clinical signs of disease (negative performance score) and histopathological parameters in the colon such as erosion/ulceration and the overall inflammation score but no negative effects were observed on systemic health or small intestinal permeability. Importantly, dietary meat also caused a potential beneficial reduction in the colonic expression of the pro-inflammatory cytokines IL-17A and IL-6, the pro-inflammatory enzyme PTGS2 and in the chemokine IL-8. The present study emphasizes the potential of diet to modulate mucosal inflammation and that a red meat diet might be a risk factor for the development of inflammatory bowel disease. |
| first_indexed | 2024-03-10T19:16:44Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T19:16:44Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-2451470ab32043c3a4648af5aeade3852023-11-20T03:19:41ZengMDPI AGNutrients2072-66432020-06-01126172810.3390/nu12061728Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene ExpressionTina S. Nielsen0Marlene Fredborg1Peter K. Theil2Yuan Yue3Lærke V. Bruhn4Vibeke Andersen5Stig Purup6Department of Animal Science, Aarhus University, DK-8830 Tjele, DenmarkDepartment of Animal Science, Aarhus University, DK-8830 Tjele, DenmarkDepartment of Animal Science, Aarhus University, DK-8830 Tjele, DenmarkDepartment of Animal Science, Aarhus University, DK-8830 Tjele, DenmarkFocused Research Unit for Molecular Diagnostics and Clinical Research, IRS-Centre Soenderjylland, University Hospital of Southern Denmark, DK-6200 Aabenraa, DenmarkFocused Research Unit for Molecular Diagnostics and Clinical Research, IRS-Centre Soenderjylland, University Hospital of Southern Denmark, DK-6200 Aabenraa, DenmarkDepartment of Animal Science, Aarhus University, DK-8830 Tjele, DenmarkDiet plays a substantial role in the pathogenesis and management of ulcerative colitis (UC), and epidemiologic studies indicate an association between red meat intake and increased risk of UC development. Therefore, we evaluated the effect of a red meat diet on dextran sulfate sodium (DSS)-induced colitis in pigs. Weaned pigs (42 days old) were fed either a control diet or a diet substituted with 15% minced, cooked and dried beef from experimental day 0 to 14. From day 14 to 18, half of the pigs on each diet received a daily oral dose of DSS. Dietary red meat aggravated the severity of colitis based on clinical signs of disease (negative performance score) and histopathological parameters in the colon such as erosion/ulceration and the overall inflammation score but no negative effects were observed on systemic health or small intestinal permeability. Importantly, dietary meat also caused a potential beneficial reduction in the colonic expression of the pro-inflammatory cytokines IL-17A and IL-6, the pro-inflammatory enzyme PTGS2 and in the chemokine IL-8. The present study emphasizes the potential of diet to modulate mucosal inflammation and that a red meat diet might be a risk factor for the development of inflammatory bowel disease.https://www.mdpi.com/2072-6643/12/6/1728dietinflammatory bowel diseaseporcinedextran sulfate sodiuminflammationhistology |
| spellingShingle | Tina S. Nielsen Marlene Fredborg Peter K. Theil Yuan Yue Lærke V. Bruhn Vibeke Andersen Stig Purup Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression Nutrients diet inflammatory bowel disease porcine dextran sulfate sodium inflammation histology |
| title | Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression |
| title_full | Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression |
| title_fullStr | Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression |
| title_full_unstemmed | Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression |
| title_short | Dietary Red Meat Adversely Affects Disease Severity in a Pig Model of DSS-Induced Colitis Despite Reduction in Colonic Pro-Inflammatory Gene Expression |
| title_sort | dietary red meat adversely affects disease severity in a pig model of dss induced colitis despite reduction in colonic pro inflammatory gene expression |
| topic | diet inflammatory bowel disease porcine dextran sulfate sodium inflammation histology |
| url | https://www.mdpi.com/2072-6643/12/6/1728 |
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