Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

ABSTRACT Trypanosomatids (order Kinetoplastida), including the human pathogens Trypanosoma cruzi (agent of Chagas disease), Trypanosoma brucei, (African sleeping sickness), and Leishmania (leishmaniasis), affect millions of people and animals globally. T. cruzi is considered one of the least studied...

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Bibliographic Details
Main Authors: Lia Carolina Soares Medeiros, Lilith South, Duo Peng, Juan M. Bustamante, Wei Wang, Molly Bunkofske, Natasha Perumal, Fernando Sanchez-Valdez, Rick L. Tarleton
Format: Article
Language:English
Published: American Society for Microbiology 2017-12-01
Series:mBio
Subjects:
CRISPR
Cas9
Leishmania
SaCas9
Trypanosoma
Trypanosoma brucei
Online Access:https://journals.asm.org/doi/10.1128/mBio.01788-17

Internet

https://journals.asm.org/doi/10.1128/mBio.01788-17

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