CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis

CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis

Abstract CRISPR/Cas9 pooled screening permits parallel evaluation of comprehensive guide RNA libraries to systematically perturb protein coding sequences in situ and correlate with functional readouts. For the analysis and visualization of the resulting datasets, we develop CRISPRO, a computational...

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Bibliographic Details
Main Authors: Vivien A. C. Schoonenberg, Mitchel A. Cole, Qiuming Yao, Claudio Macias-Treviño, Falak Sher, Patrick G. Schupp, Matthew C. Canver, Takahiro Maeda, Luca Pinello, Daniel E. Bauer
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Genome Biology
Subjects:
CRISPR screen
Protein structure
Tiling
Saturating
Comprehensive
Mutagenesis
Online Access:http://link.springer.com/article/10.1186/s13059-018-1563-5

Internet

http://link.springer.com/article/10.1186/s13059-018-1563-5

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