Extraction-free whole transcriptome gene expression analysis of FFPE sections and histology-directed subareas of tissue.

Extraction-free whole transcriptome gene expression analysis of FFPE sections and histology-directed subareas of tissue.

We describe the use of a ligation-based targeted whole transcriptome expression profiling assay, TempO-Seq, to profile formalin-fixed paraffin-embedded (FFPE) tissue, including H&E stained FFPE tissue, by directly lysing tissue scraped from slides without extracting RNA or converting the RNA to...

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Bibliographic Details
Main Authors: Christy L Trejo, Miloš Babić, Elliot Imler, Migdalia Gonzalez, Sergei I Bibikov, Peter J Shepard, Harper C VanSteenhouse, Joanne M Yeakley, Bruce E Seligmann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0212031

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https://doi.org/10.1371/journal.pone.0212031

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