Protocol for formation, staining, and imaging of 3D breast cancer models using MicroTissues mold systems

Summary: The use of 3D cancer models to study therapy response has a great advantage over conventional 2D cell culture. Here, we present an optimized protocol for breast cancer spheroid and rod-shaped microtissue formation using MicroTissues mold systems. We describe steps for cast formation and tre...

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Bibliographic Details
Main Authors: Ben Haspels, Maayke M.P. Kuijten
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724004155