The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing

<p>Abstract</p> <p>Background</p> <p>The use of RNAi to analyse gene function <it>in vitro </it>is now widely applied in biological research. However, several difficulties are associated with its use <it>in vivo</it>, mainly relating to inefficie...

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Bibliographic Details
Main Authors: Lasham Annette, Coppieters Natacha, Herbert Mike, Cao Helen, Reid Glen
Format: Article
Language:English
Published: BMC 2011-05-01
Series:BMC Research Notes
Online Access:http://www.biomedcentral.com/1756-0500/4/148