Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway
The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2011-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700004&lng=en&tlng=en |
| _version_ | 1828239275287642112 |
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| author | Xu Xiangming Qian Yun Zhang Guoliang Lin Jianjiang Teng Lisong |
| author_facet | Xu Xiangming Qian Yun Zhang Guoliang Lin Jianjiang Teng Lisong |
| author_sort | Xu Xiangming |
| collection | DOAJ |
| description | The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results in cell migration and initiates changes in the morphology of RON-cDNA-transfected MDCK cells. The absence of E-cadherin, the presence of vimentin and an increase in Snail were observed in RE7 cells, which were derived from MDCK cells transfected with wt-RON, compared with MDCK cells. Stimulation of RE7 cells with MSP resulted in increased migration (about 69% of the wounded areas were covered) as well as increased activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and glycogen synthase kinase-3β (GSK-3β; the percent of the activation ratio was 143.6/599.8% and 512.4%, respectively), which could be inhibited with an individual chemical inhibitor PD98059 (50 μM) specific to MAPK/ERK kinase (the percent inhibition was 98.9 and 81.2%, respectively). Thus, the results indicated that RON protein could mediate EMT in MDCK cells via the Erk1/2 pathway. Furthermore, GSK-3β regulates the function of Snail in controlling EMT by this pathway. |
| first_indexed | 2024-04-12T21:20:02Z |
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| id | doaj.art-24d5528fa3dc4d05bd201e5c989c042f |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-12T21:20:02Z |
| publishDate | 2011-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-24d5528fa3dc4d05bd201e5c989c042f2022-12-22T03:16:19ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2011-07-0144763464110.1590/S0100-879X2011000700004S0100-879X2011000700004Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathwayXu Xiangming0Qian Yun1Zhang Guoliang2Lin Jianjiang3Teng Lisong4Zhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityThe epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results in cell migration and initiates changes in the morphology of RON-cDNA-transfected MDCK cells. The absence of E-cadherin, the presence of vimentin and an increase in Snail were observed in RE7 cells, which were derived from MDCK cells transfected with wt-RON, compared with MDCK cells. Stimulation of RE7 cells with MSP resulted in increased migration (about 69% of the wounded areas were covered) as well as increased activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and glycogen synthase kinase-3β (GSK-3β; the percent of the activation ratio was 143.6/599.8% and 512.4%, respectively), which could be inhibited with an individual chemical inhibitor PD98059 (50 μM) specific to MAPK/ERK kinase (the percent inhibition was 98.9 and 81.2%, respectively). Thus, the results indicated that RON protein could mediate EMT in MDCK cells via the Erk1/2 pathway. Furthermore, GSK-3β regulates the function of Snail in controlling EMT by this pathway.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700004&lng=en&tlng=enRONEpithelial-mesenchymal transitionMAPKErk |
| spellingShingle | Xu Xiangming Qian Yun Zhang Guoliang Lin Jianjiang Teng Lisong Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway Brazilian Journal of Medical and Biological Research RON Epithelial-mesenchymal transition MAPK Erk |
| title | Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway |
| title_full | Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway |
| title_fullStr | Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway |
| title_full_unstemmed | Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway |
| title_short | Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway |
| title_sort | mechanisms of ron mediated epithelial mesenchymal transition in mdck cells through the mapk pathway |
| topic | RON Epithelial-mesenchymal transition MAPK Erk |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700004&lng=en&tlng=en |
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