Rapid in vitro splicing of coding sequences from genomic DNA by isothermal recombination reaction-based PCR

Rapid in vitro splicing of coding sequences from genomic DNA by isothermal recombination reaction-based PCR

Cloning of coding sequence (CDS) is an important step for gene function research. Here, we reported a simple and efficient strategy for assembling multiple-exon into an intron-free CDS from genomic DNA (gDNA) by an isothermal recombination reaction-based PCR (IRR-PCR) method. As an example, a 2067-b...

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Bibliographic Details
Main Authors: Wenxuan Chen, Dongchang Zeng, Rongxin Shen, Xingliang Ma, Qunyu Zhang, Letian Chen, Yao-Guang Liu, Qinlong Zhu
Format: Article
Language:English
Published: Taylor & Francis Group 2016-09-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Coding sequences
DNA fragments
splicing
genomic DNA
isothermal recombination reaction-based PCR
IRR-PCR
Online Access:http://dx.doi.org/10.1080/13102818.2016.1191374

Internet

http://dx.doi.org/10.1080/13102818.2016.1191374

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