Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen <em>Eubacterium limosum</em>

Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen <em>Eubacterium limosum</em>

Targeted mutations in the anaerobic methylotroph <i>Eubacterium limosum</i> have previously been obtained using CRISPR-based mutagenesis methods. In this study, a RelB-family toxin from <i>Eubacterium callanderi</i> was placed under the control of an anhydrotetracycline-sensi...

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Bibliographic Details
Main Authors: James Millard, Alexander Agius, Ying Zhang, Philippe Soucaille, Nigel Peter Minton
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:Microorganisms
Subjects:
<i>Eubacterium limosum</i>
<i>Eubacterium callanderi</i>
toxin–antitoxin systems
RelBE
CRISPR
gene editing
Online Access:https://www.mdpi.com/2076-2607/11/5/1256

Internet

https://www.mdpi.com/2076-2607/11/5/1256

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